SUMMARY OF RESEARCH DIRECTIONS
Prof. Mark A. Eiteman has worked in research and teaching related to metabolic engineering and fermentation technology for over 20 years. A focus of his research is developing microbial processes to generate fuels and chemicals. Through an understanding of microbial regulatory mechanisms and redox balance constraints, strains and processes are developed to maximize product formation. Examples of products of recent interest include succinic acid, lactic acid, pyruvic acid and 5-aminolevulinate.
Dr. Eiteman is also presently developing technology to remove the microbial growth inhibitor acetic acid selectively from lignocellulosic hydrolysates, and to convert the resulting mixed sugar stream into ethanol. Through his research, he has authored or coauthored over 60 peer reviewed journal articles. He teaches a mass transfer course, as well as senior and graduate level engineering courses in biochemical engineering and bioseparations at the University of Georgia. For more information, see Dr. Eiteman's Lab website.
Ravi R. Gokarn, Mark A. Eiteman, Elliot Altman, "Metabolically Engineered E. coli for Enhanced Production of Oxaloacetate-Derived Biochemicals." U.S. Patent 6,455,284 (2002).
Mark A. Eiteman and Elliot Altman, "Substrate-Selective Co-Fermentation Process." Biological method for conversion of a lignocellulosic hydrolysate into a desired biochemical product. Use of a plurality of substrate-selective cells allows different sugars in a complex mixture to be consumed concurrently and independently. The method can be readily extended to remove inhibitory compounds from hydrolysate.
E. Altman, M. A. Eiteman, "The Potential for Using Escherichia coli and Other Organisms to Produce Recombinant Ingredients for the Cosmetic Industry," in Microorganisms and Cosmetics, Anthony O'Lenick Jr. (ed.) Allured Books, Carol Stream, IL, pp. 385-394, 2009.
M. A. Eiteman, S. A. Lee, R. Altman, E. Altman, "A substrate-selective co-fermentation strategy with Escherichia coli produces lactate by simultaneously consuming xylose and glucose," Biotechnology and Bioengineering, 102:822-827 (2009).
Y. Zhu, M. A. Eiteman, R. Altman, E. Altman, "High glycolytic flux improves pyruvate production by a metabolically engineered Escherichia coli strain," Applied and Environmental Microbiology, 74(21):6649-6655 (2008).
Y. Zhu, M. A. Eiteman, E. Altman, "Indirect monitoring of acetate exhaustion and cell recycle improve lactate production by non-growing Escherichia coli," Biotechnology Letters, 30:1943-1946 (2008).
M. A. Eiteman, S.A. Lee, E. Altman, "A co-fermentation strategy to consume sugar mixtures effectively," Journal of Biological Engineering, 2:3 (2008).
S.K. Brandon, M. A. Eiteman, K. Patel, M.M. Richbourg, D.J. Miller, W.F. Anderson, J.D. Peterson, "Hydrolysis of Tifton 85 Bermudagrass in a Pressurized Batch Hot Water Reactor," Journal of Chemical Technology and Biotechnology, 83:505-512 (2008).
G.N. Vemuri, M. A. Eiteman, J.E. McEwen, L. Olsson, J. Nielsen, "Increasing NADH oxidation reduces overflow metabolism in Saccharomyces cerevisiae," Proceedings of the National Academy of Sciences, 104(7), 2402-2407 (2007).
Y. Zhu, M. A. Eiteman, K. DeWitt, E. Altman, "Homolactate Fermentation by Metabolically Engineered Escherichia coli," Applied and Environmental Microbiology, 73(2), 456-464 (2007).
M. A. Eiteman, and E. Altman. Overcoming acetate in Escherichia coli recombinant protein fermentations. Trends in Biotechnology 24: 530-536, (2006).
G.M. Smith, S.A. Lee, K.C. Reilly, M. A. Eiteman, E. Altman, "Fed-Batch Two-Phase Production of Alanine by a Metabolically Engineered Escherichia coli," Biotechnology Letters, 28, 1695-1700 (2006).
G.N. Vemuri, E. Altman, D.P. Sangurdekar, A.B. Khodursky, M. A. Eiteman, "Overflow Metabolism in Escherichia coli During Steady-State Growth: Transcriptional Regulation and Effect of the Redox Ratio," Applied and Environmental Microbiology, 72(5), 3652-3661 (2006).
G.N. Vemuri, M. A. Eiteman, E. Altman, "Increased Recombinant Protein Production in Escherichia coli Strains with Overexpressed Water-Forming NADH Oxidase and a Deleted ArcA Regulatory Protein," Biotechnology and Bioengineering, 94(3), 538-542 (2006).
G.N. Vemuri, T.A. Minning, E. Altman, M. A. Eiteman, "Physiological Response of Central Metabolism in Escherichia coli to Deletion of Pyruvate Oxidase and Introduction of Heterologous Pyruvate Carboxylase," Biotechnology and Bioengineering, 90(1), 64-76 (2005).
M. Lee, G. Smith, M. A. Eiteman, E. Altman, "Aerobic Production of Alanine by Escherichia coli aceF ldhA Mutants Expressing the Bacillus sphaericus alaD gene," Applied Microbiology and Biotechnology, 65, 56-60 (2004).
L. Xie, D. Hall, M. A. Eiteman, E. Altman, "Optimization of Recombinant Aminolevulinate Synthase Production in Escherichia coli using Factorial Design," Applied Microbiology and Biotechnology, 63, 267-273 (2003).
L. Xie, M. A. Eiteman, E. Altman, "Production of 5-Aminolevulinic Acid by an Escherichia coli Aminolevulinate Dehydratase Mutant that Overproduces Rhodobacter sphaeroides Aminolevulinate Synthase," Biotechnology Letters, 25, 1751-1755 (2003).